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  • International Journal of Microbial Resource Technology
  • IJMRT
  • Our manuscripts areas
  • International Journal of Microbial Resource Technology

    ISSN 2278-3822
    Frequency: Quarterly
    We guarantee rapid publication. The journal is comprehensive.The journal is driven by international, well-known Editors.It provides an easy-to-use online submission and tracking system. It also provides innovative results and new imaging technologies.The journal regularly brings up-to-date with focused/current topics.Since the very ancient time man is exploiting Microorganisms as natural resources

  • IJMRT

    ISSN 2278 – 3822
    Frequency: Quarterly
    International Journal of Microbial Resource Technology (IJMRT) Guarantees rapid publication.This is comprehensive.The journal is driven by international, well-known Editors.It provides an easy-to-use online submission and tracking system. It also provides innovative results and new imaging technologies.

  • Our manuscripts areas

    Microbiology, Ecology, Genetics and Cell Biology,Cell development, Microbial interactions, Advanced/new methodologies, Novel Molecular Biology methods , Microbial Physiology and Metabolism.

Detail of Issue

  • Isolation, Identification and Characterization of Efficient Cellulolytic Fungi from Natural Resources

    Rahna K. Rathnan, Preethi Nair and Balasaravanan, T.
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Abstract

The isolation and screening of efficient cellulase producing fungi from natural sources was studied in this work. This research was aimed to isolate cellulolytic fungi with overproduction of cellulase components. Efficient Cellulase-producing fungi were isolated from different samples like soil, compost, decayed lignocellulosic waste etc using different isolation strategies. A novel methodology of isolation and primary screening of cellulolytic fungi was adopted by exposing carboxy methyl cellulose agar, which is a selective media for cellulolytic microorganisms, directly in the environment which is rich with cellulolytic microbes. Among the various isolates obtained from different environment, five different fungi were selected depending upon the diameter of clear zone produced in Carboxy methyl cellulose agar for further screening in liquid media and one potent strain NASC3 was identified as efficient cellulolytic fungi. Molecular identification of strain NASC3 was done by PCR amplification of 18s rDNA region using primers ITS4 and ITS5. The amplified products were sequenced and analyzed using ClustalW .The phylogenetic analysis of strain NASC3 showed highest homology with Penicillium citrinum.These Cellulase positive isolates may be an integral part of future work to develop good cellulases or produce efficient cellulase producing systems such as microbial consortia which can be used for industry. Isolation and characterization may also provide a good starting point for the discovery of such beneficial enzymes.

 

Key words: Cellulase, Fungi, Molecular identification, 18S rRNA, P. citrinum