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  • International Journal of Microbial Resource Technology
  • IJMRT
  • Our manuscripts areas
  • International Journal of Microbial Resource Technology

    ISSN 2278-3822
    Frequency: Quarterly
    We guarantee rapid publication. The journal is comprehensive.The journal is driven by international, well-known Editors.It provides an easy-to-use online submission and tracking system. It also provides innovative results and new imaging technologies.The journal regularly brings up-to-date with focused/current topics.Since the very ancient time man is exploiting Microorganisms as natural resources

  • IJMRT

    ISSN 2278 – 3822
    Frequency: Quarterly
    International Journal of Microbial Resource Technology (IJMRT) Guarantees rapid publication.This is comprehensive.The journal is driven by international, well-known Editors.It provides an easy-to-use online submission and tracking system. It also provides innovative results and new imaging technologies.

  • Our manuscripts areas

    Microbiology, Ecology, Genetics and Cell Biology,Cell development, Microbial interactions, Advanced/new methodologies, Novel Molecular Biology methods , Microbial Physiology and Metabolism.

Detail of Issue

  • Chromosomally encoded phenol hydroxylase gene for degradation of phenol by Pseudomonas aeruginosa MTCC 4996

    Kotresha D. and Vidyasagar G.M
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Abstract

Phenol considered as one of most important industrial pollutant that pose severe threats to human health and the environment. The aim of this study was molecular characterization of new strain capable of phenol degradation. Phenol was found to be metabolized via ortho ring cleavage pathway by phenol hydroxylase and catechol 1, 2-dioxygenase.  Further phenol hydroxlase was partial purified and specific activity was increased 7.8 fold by using DEAE-sepharose. P. aeruginosa MTCC 4996 plasmid cured cells were able to utilize phenol as a carbon source, it is confirmed that the gene responsible for phenol degradation is located on chromosome.

 

Keywords : Pseudomonas aeruginosa MTCC 4996, Phenol degradation, Phenol hydroxylase, Catechol 1, 2-dioxygenase, Ortho ring cleavage pathway